Sequence Inputs
Gateway Cloning Workflow
Upload Entry clones or enter raw sequences. Raw sequences receive attB primers for BP reaction. The LR reaction combines Entry clones with pDEST to create the final Expression clone.
Destination Vector (pDEST)
Upload pDEST vector with attR4 and attR3 sites
No vector loaded
5' Element (Promoter)
pDONR P4-P1R Entry Clone (attL4-insert-attR1) or raw sequence
Length: 0 bp
Middle Element (Gene of Interest)
pDONR 221 Entry Clone (attL1-insert-attL2) or raw sequence
Length: 0 bp
3' Element (Terminator/Tag)
pDONR P2R-P3 Entry Clone (attR2-insert-attL3) or raw sequence
Length: 0 bp
Gateway Reaction Workflow
Enter sequences and click "Generate Assembly & Primers" to see the reaction workflow
Expression Clone Sequence
Final expression clone sequence will appear here
Translation (Longest ORF in Insert)
Translation will appear here after assembly
Statistics
Total Assembly Size:
0 bp
GC Content:
0%
Molecular Weight:
0 Da
attB Site Reference
| Site | Sequence (5'->3') |
|---|---|
| attB4 | GGGGACAACTTTGTATAGAAAAGTTG |
| attB1r | GGGGACTGCTTTTTTGTACAAACTTG |
| attB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCT |
| attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGT |
| attB2r | GGGGACAGCTTTCTTGTACAAAGTGG |
| attB3 | GGGGACAACTTTGTATAATAAAGTTG |
PCR Primers
Primers for BP reaction will be generated after assembly